主 办:北 京 中 医 药 大 学
ISSN 2095-6606 CN 10-1157/R

现代中医临床 ›› 2020, Vol. 27 ›› Issue (4): 13-16.doi: 10.3969/j.issn.2095-6606.2020.04.003

• 临床研究 • 上一篇    下一篇

银屑病血瘀证患者外周血辅助性T细胞表达差异的研究*

张益生1, 陈若曦1, 赵雅静2,3, 杭小涵1, 李元文2,3, 孙占学2,3#   

  1. 1 北京中医药大学 北京 100029;
    2 北京中医药大学东方医院皮肤科;
    3 北京中医药大学皮肤病研究所
  • 收稿日期:2019-11-17 发布日期:2020-07-30
  • 通讯作者: #孙占学,男,博士,主任医师,硕士生导师,E-mail:sunzhanxue@163.com
  • 作者简介:张益生,女,在读硕士生
  • 基金资助:
    *丰台区卫生健康系统科研项目(No.2019-81),北京市自然科学基金资助项目(No.7192116),北京中医药大学东方医院“1166”人才培养项目(No.030903010323)

Study on differential expression of T helper cells in psoriasis with syndrome of blood stasis*

Zhang Yisheng1, Chen Ruoxi1, Zhao Yajing2,3, Hang Xiaohan1, Li Yuanwen2,3, Sun Zhanxue2,3#   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 Department of Dermatology, Dongfang Hospital, Beijing University of Chinese Medicine;
    3 Dermatology Research Institution, Beijing University of Chinese Medicine
  • Received:2019-11-17 Published:2020-07-30

摘要: 目的 观察银屑病血瘀证患者外周血辅助性T细胞(Th)表达的差异。方法 选择银屑病血瘀证患者30例为银屑病组。同时选取30例健康志愿者作为对照组。采用酶联免疫吸附测定(ELISA)法分别检测2组外周血γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-17A(IL-17A)、白细胞介素-10(IL-10)、白细胞介素-22(IL-22)水平,采用银屑病皮损面积和严重程度指数(PASI)评分评价银屑病组病情严重程度。对2组IFN-γ,IL-4,IL-17A,IL-10,IL-22水平进行比较,观察银屑病组细胞因子与PASI评分之间的相关性。结果 银屑病组IFN-γ,IL-17A较对照组增高,差异均有统计学意义(P<0.05)。IL-4,IL-10,IL-22与对照组比较差异均无统计学意义(P>0.05)。银屑病组IL-17A/IL-10比值与PASI评分呈正相关(r=0.455, P<0.05),IFN-γ,IL-4,IL-17A,IL-10,IL-22以及IFN-γ/IL-4比值与PASI评分均无相关性(P>0.05)。结论 Th1,Th17可能在血瘀证中处于优势应答状态,Th2、调节性T细胞(Treg)、Th22可能无异常表达。Th17/Treg漂移可能在血瘀证中占据主导地位。

关键词: 银屑病, 血瘀证, 免疫功能, 细胞因子, 相关性

Abstract: Objective To observe the different expression of T helper cells(Th) in serum of psoriasis with syndrome of blood stasis. Methods Levels of interferon-gamma (INF-γ), interleukin-4( IL-4 ),interleukin-17A( IL-17A ),interleukin-10( IL-10 ) and interleukin-22( IL-22) in serum of 30 patients with psoriasis with syndrome of blood stasis and 30 healthy controls were detected by enzyme-linked immuno sorbent assay(ELISA). Psoriasis area severity index(PASI) scores were calculated to assess the severity.Relevances were analysed with the cytokines and the PASI scores. Results IFN-γ and IL-17A were significantly higher in 30 patients than in healthy controls(P<0.05).The differences of IL-4,IL-10 and IL-22 between the two groups were not statistically significant(P>0.05).IL-17/IL-10 was positively related to the PASI scores significantly(r=0.455,P<0.05).There was no correlation between serum IFN-γ,IL-4,IL-17A,IL-10,IL-22,IFN-γ/IL-4 and PASI scores(P>0.05). Conclusions Th1 and Th17 may be in the dominant response state in psoriasis with syndrome of blood stasis. While Th2,T regulatory cell(Treg) and Th22 may not have abnormal expression.Th17/Treg drift may play a dominant role in psoriasis with syndrome of blood stasis.

Key words: psoriasis, syndrome of blood stasis, immune function, cytokines, relevances

中图分类号: 

  • R275