Effect of preconditioning with bian-stone patches at acupoints on myocardial injury in rats with acute hypobaric hypoxia

YAN Yingjie; HOU Zhongwei; LI Xiaoya; WU Chunhua; LI Hang; WANG Haidong; WANG Mengjie

doi:10.3969/j.issn.2095-6606.2023.05.010

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Effect of preconditioning with bian-stone patches at acupoints on myocardial injury in rats with acute hypobaric hypoxia

TCM Clinical and Basic Studies | 更新时间：2023-10-12

- Effect of preconditioning with bian-stone patches at acupoints on myocardial injury in rats with acute hypobaric hypoxia
- Modern Chinese Clinical Medicine Vol. 30, Issue 5, Pages: 53-58(2023)
- 作者机构：
  
  1.北京中医药大学针灸推拿学院　北京　102488
  2.解放军总医院第六医学中心
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.2095-6606.2023.05.010
  CLC： R259
- Received：11 October 2022，
  
  Published：30 September 2023
- 稿件说明：
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YAN Yingjie, HOU Zhongwei, LI Xiaoya, et al. Effect of preconditioning with bian-stone patches at acupoints on myocardial injury in rats with acute hypobaric hypoxia[J]. Modern Chinese Clinical Medicine, 2023, 30(5): 53-58.
DOI：

YAN Yingjie, HOU Zhongwei, LI Xiaoya, et al. Effect of preconditioning with bian-stone patches at acupoints on myocardial injury in rats with acute hypobaric hypoxia[J]. Modern Chinese Clinical Medicine, 2023, 30(5): 53-58. DOI： 10.3969/j.issn.2095-6606.2023.05.010.

摘要

目的

探究砭贴预处理内关、太渊、人迎穴对急性低压低氧模型大鼠心肌损伤和氧化应激程度的影响。

方法

将36只SD雄性大鼠，随机分为空白组、模型组、砭贴组、药物组，每组9只。空白组和模型组不进行干预，砭贴组大鼠每日在双侧内关、太渊、人迎穴处贴砭贴2 h，药物组大鼠按照280 mg/(kg·d)剂量进行大株红景天胶囊灌胃。干预14 d后，除空白组大鼠外，其余3组大鼠均需放入氧舱中持续24 h以模拟高原6000 m海拔环境。出舱后进行腹主动脉取血、心肌组织取材。用ELISA法测定大鼠血清和心肌中肌酸激酶同工酶(creatine kinase isoenzymes

CK-MB)、肌钙蛋白I(cardiac troponin I

cTnI)、脑钠肽(B-type natriuretic peptide

BNP)表达，测定心肌组织丙二醇(malondialdehyde

MDA)、过氧化氢(hydrogen peroxide

)、低氧诱导因子(hypoxia-inducible factor

HIF)－1α表达，HE染色观察心肌组织病理学改变并测定心肌组织含水量。

结果

病理切片显示模型组大鼠心肌组织细胞出现溶解、断裂、细胞间隙增宽的现象。砭贴组和药物组较模型组病理改变较轻，缺氧损伤表现不明显。模型组较空白组心肌含水量增多(

0.05)，血清CK-MB、cTnI、BNP水平升高(

0.05)，心肌中CK-MB、cTnI、BNP、H

、MDA、HIF－1α水平升高(

0.05)；砭贴组较空白组心肌MDA水平降低(

0.05)。砭贴组较模型组心肌含水量降低(

0.05)，血清CK-MB、cTnI、BNP水平降低(

0.05)，心肌CK-MB、cTnI、BNP、H

、MDA、HIF－1α水平降低(

0.05)；药物组较模型组血清CK-MB、cTnI水平降低(

0.05)，心肌CK-MB、cTnI、BNP、H

、MDA水平降低(

0.05)，心肌含水量、血清BNP水平、心肌HIF－1α水平有下降趋势。砭贴组较药物组，心肌BNP水平降低(

0.05)。

结论

砭贴预处理内关、太渊、人迎穴可改善模型大鼠心肌缺氧状态，降低氧化应激程度，减轻低压低氧环境诱导的心肌损伤，为急性高原心脏病提供了新的治疗思路和防治措施。

Abstract

Objective

To observe the effects of preconditioning with bian-stone patches at acupoints Neiguan (PC 6)

Taiyuan (LU 9)

and Renying (ST 9) on myocardial injury and oxidative stress in rats with acute hypobaric hypoxia.

Methods

36 male SD rats were randomly divided into the blank group

model group

patch group and drug group

with 9 rats in each. The blank group and model group were not given any interventions. Bian-stone patches were applied on the rats in the patch group at Taiyuan

Neiguan

and Renying points on both sides of the body and kept in place for 2 hours every day. Rats in the drug group were given 280 mg/kg of Rhodiola Capsule daily. After 14 days of intervention

all groups except the blank group entered the oxygen chamber which simulated the plateau environment at an altitude of 6000 m and stayed there for 24 hours. After they left the chamber

blood samples from the abdominal aorta and myocardial tissues were collected from the rats. The expressions of creatine kinase-MB (CK-MB)

cardiac troponin I (cTnI) and brain natriuretic peptide (BNP) in the serum and myocardium

and also the expressions of malondialdehyde (MDA)

hydrogen peroxide (H

) and hypoxia-inducible factor-1α (HIF-1α) in the myocardial tissues were determined by ELISA. The pathological changes of the myocardial tissues were observed and their water content were measured by HE staining.

Results

Pathological sections showed that the myocardial tissue cells in the model group were dissolved

broken and intercellular space widened. The pathological changes in the patch and drug groups were milder than those in the model group

and the hypoxic injury was not significant. Compared with the blank group

the myocardial water content in the model group was increased (

0.05)

the serum levels of CK-MB

cTnI and BNP were elevated (

0.05)

and the myocardial levels of CK-MB

cTnI

BNP

MDA and HIF-1α were raised (

0.05). The myocardial level of MDA in the patch group decreased (

0.05). Compared with model group

the myocardial water content in the patch group was increased (

0.05)

serum levels of CK-MB

cTnI and BNP were lowered (

0.05)

and the myocardial levels of CK-MB

cTnI

BNP

MDA and HIF-1α were reduced (

0.05). The levels of serum CK-MB and cTnI in drug group decreased (

0.05)

the levels of myocardial CK-MB

cTnI

BNP

and MDA were reduced (

0.05)

and the levels of myocardial water content

serum BNP and HIF-1α were decreased. Compared with drug group

the myocardial level of BNP in the patch group was lowered (

0.05).

Conclusion

Preconditioning with bian-stone patches at acupoints Neiguan

Taiyuan and Renying can improve the myocardial hypoxia of model rats

reduce the degree of oxidative stress

and alleviate the myocardial injury induced by hypobaric hypoxia

providing a new treatment idea and prevention measures for acute high altitude heart disease.

关键词

Keywords

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